Physicochemical Properties of Hesperidin Nanocrystal

Hesperidin nanocrystals was generated by high pressure homogenization. The physicochemical properties of a spray dried hesperidin nanocrystals were investigated including to re-dispersion, crystalline state, kinetic solubility, dissolution velocity. Hesperidin nanosuspension with concentration of 10% (w/v) was dried by a Mini Spray-dryer B-190. Photon correlation spectroscopy (PCS) and laser diffraction (LD) were employed to determine the particle size. DSC and X-ray diffraction were used to study crystalline state. The saturation solubility and dissolution were determined at certain time points at 25oC. Spray-dried hesperidin nanocrystals could be redispersed in water completely. The size average and polydispersity index (PI) were 396 nm and 0.555 after redispersion. The d50% of nanocrystals was 0.370 μm. The crystalline state showed that the high pressure homogenization process did not change the crystaline state. Saturation solubility of spray-dried hesperidin nanocrystals in water was 87.2 μg/ml and higher than hesperidin raw material. Hesperidin nanocrystals was dissolved completely within 15 minutes in various media. Whereas hesperidin raw materials after 15 minutes was dissolved maximum only 37%. Spray-dried hesperidin nanocrystals could provide superior physicochemical properties. Spray-dried hesperidin nanocrystals increased the saturation solubility and especially the dissolution velocity in comparison to raw material, i.e. drug delivery improved in case dissolution velocity is the rate limiting step.